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Single Nucleotide Polymorphisms: Methods and Protocols (Methods in Molecular Biology, 578) In recent years, single nucleotide
polymorphisms have received increased and special
attention in a rapidly developing field of personalized
medicine and drug treatment. Comprising more than eighty
percent of all known polymorphisms, single
nucleotide polymorphisms (SNPs)
are primarily responsible for phenotypic differences
between individuals, and have been suggested to affect the
development of diseases in humans and the response to drug
treatment and environmental stress. In Single
Nucleotide Polymorphisms: Methods and
Protocols, Second Edition, expert researchers explore the
latest advances in this area, highlighting the substantial
progress that has been made in SNP
genotyping, examining recent developments in
high-throughput genotyping approaches, and exploring our
new understanding of the impact of SNPs on gene function.
Chapters address the impact of SNPs on phenotype, examine
SNP databases, look at methods that have been applied for
SNP bioinformatics discovery and analysis, and discuss
advanced experimental approaches used for SNP detection.
Composed in the highly successful Methods in Molecular
Biology™ series format, each chapter contains a brief
introduction, step-by-step methods, a list of necessary
materials, and a Notes section which shares tips on
troubleshooting and avoiding known pitfalls.
Chemistry of Nucleosides and Nucleotides: Volume 3 This third of four
volumes continues the work of its well-received
predecessors by providing comprehensive articles on
specific lines of research. All volumes are written with
medicinal chemists, organic chemists, physical chemists,
and biological chemists in mind. Additionally, with the
spate of recent research on the anticancer, antiviral,
and antiparasitic properties of nucleosides and nucleotides,
the volumes will interest oncologists, virologists, and
pharmacologists
Genetic Consequences of Nucleotide Pool Imbalance (Basic Life Sciences, 31) This book is concerned
with the genetic consequences of nucleotide
pool imbalance. There are a number of ways in which this
state can be achieved and the majority of papers in this
volume de scribe the effects of nucleotide pool
imbalance on DNA replication and repair, and the
induction of gene mutations in various organisms. This
book grew out of a Conference on "Genetic Consequences
of Nucleotide Pool Imbalance" which was held at the
National Institute of Environmental Health Sciences on
May 9-11, 1983. The Organizing Com mittee consisted of
Drs. Diana Anderson (BIBRA), Robert Haynes (York
University), Bernard Kunz (NIEHS), Barry Glickman
(NIEHS), and Frederick J. de Serres (NIEHS), Chairman.
The meeting was org- ized by the staff of the Office of
the Associate Director for Genetics and was financed
solely by the National Institute of Environmental Health
Sciences. Frederick J. de Serres, Ph.D. Associate
Director for Genetics v CONTENTS SECTION I. INTRODUCTION
Molecular Mechanisms in Genetic Stability and Change: 1
The Role of Deoxyribonucleotide Pool Balance ••••••
Robert H. Haynes SECTION II. NUCLEOTIDE
METABOLISM 25 Metabolic Effects of Poly(ADP-ribose)
Inhibitors •••••••• Kathryn M. Milam and James E.
Cleaver Ribonucleotide Reductase and Deoxyribonucleotide
33 POQ 1 s ............................................
. Peter Reichard 47 Enzymatic Channeling of DNA
Precursors •••••••
This unique advanced
textbook provides a clear and comprehensive overview of
gene delivery, gene therapy and genetic pharmacology,
with descriptions of the main gene transfer vectors and
a set of selected therapeutic applications, along with
safety considerations. The second edition features new
groundbreaking material on genome editing using the
recently discovered CRISPR/Cas9 system and on cancer
immunotherapy by CAR-T cells. It also presents the
historical milestone of gene therapy application in the
field of severe combined immunodeficiency, and other
fields of gene therapy and molecular medicine. The use
of gene transfer is exponentially growing in the
scientific and medical communities for day-to-day cell
biology experiments and swift development of gene
therapy, which is already revolutionizing medicine. In
this advanced textbook, more than 30 leading scientists
come together to explore these topics. This educational
introduction provides the background material needed to
further explore the subject as well as relevant research
literature. It is an invaluable resource to Master, PhD
or MD students, post-doctoral scientists or medical
doctors, as well as any scientist wishing to deliver a
gene or synthetic nucleotide or
develop a gene therapy strategy. The second edition's
simple and synthetic content will be of value to any
reader interested in the biological and medical
revolution derived from the elucidation of the human
genome.
Cyclic Nucleotides in the Nervous System The elucidation of the
cellular and molecular bases underlying the inte grated
function of the central nervous system, both in disease
and in health, must ultimately come from the combined
efforts of scientists from many disciplines, including
biology, chemistry, histology, pathology, physiology,
pharmacology, and psychology. Communication between
scientists from these various disciplines-vital to the
advancement of our understanding of the function of the
nervous system-has become more and more difficult in
recent years. Both increasing specialization and the
incredible increases in publications pertinent to brain
research in a wide spectrum of journals, in symposium
volumes, in monographs, in abstracts, and in reviews
contrib ute to the problems of cross-communication and
even of communication within a scientific discipline.
Research on the significance of cyclic nucleotides
to the function of nervous systems is particularly
illustrative of the communication problem. Since the
initial publications by Sutherland, Rall, and Butcher in
the late fifties and early sixties on high levels of
adenylate cyclase, phosphodiesterases, and cyclic AMP in
brain, the ensuing litera ture of this field has
expanded exponentially. At the present time, from five
to ten publications relevant to cyclic nucleotides
and the nervous system appear each week. Indeed, these
are minimal numbers based mainly on examination of
literature titles and key index words. Many articles
concerned with some aspect of central function contain,
buried within their text, experiments with or related to
cyclic nucleotides.
Nucleotide Metabolism: An Introduction Nucleotide
Metabolism: An Introduction is a textbook exclusively
focusing on the study of the aspects of nucleotide
metabolism. The book intends to present the
chemistry and metabolism of nucleotides,
one of the oldest subjects of biochemistry. The text is
divided in two parts. Part I considers the general
aspects of nucleotide metabolism such as the history of
the discovery of nucleotides;
functions of nucleotides in cells; and group-transfer
reactions during nucleotide metabolism. Part II deals
with the synthesis, formation, and conversion of purine
ribonucleotides. Biochemists, pharmacologists, and
researchers in the fields of medicine and
pharmaceuticals will find the book invaluable.
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